Determination of total flavonoid, antioxidant, active constituents and liver function enzyme of Plantago laceolata aqueous extract

Plantago laceolata considered as an ancient herb promising plant. In this work, the target of study was designed to estimate total flavonoids, anti-oxidant and chemical composition aqueous extract in vitro. Total flavonoids content was;evaluated extract, it 317.0000 ± 9.64365mg /ml. also, activity vitro assessed via evaluation reductive ability. At all concentrations plant tested (0.02, 0.04, 0.08, 0.16, 0.32 0.64 mg/ml) ability; absorbance increase significantly dependent concentration manner. The results showed contain Tannins, polysaccharide, Alkaloids,Flavonoids Polyphenolic Compounds . Also extracts counteract damage caused by methotrexate drug through reduction liver enzymes GOT, GPT ALP groups mice interactions indicated ability modulating harmful effects its when administration albino male 200mg/kg.